Coliform Group Enzvme substrate detection reagent For water testing

Item Name : Cotiform Group Enzvme   substrate   detection   reagent Character  :This product is white or light yellow particles Clarification : degree   Colorless or slightly yellow PH    7.0-7.8 Weight : 2.7士 0.5 g Storage: Long term storage, drying, sealing and avoiding light storage at 4°C - 8°C Term of Validity  : 1 years
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Products Details

Character

 This product is white or light yellow particles

Clarification degree

 Colorless or slightly yellow

PH

 7.0-7.8

Weight

 2.7士 0.5 g

Storage

 Long term storage, drying, sealing and avoiding light storage at 4°C - 8°C

Term of Validity

 1 years

Working Principle

  In the water samples containing total coliform bacteria, the target bacteria were cultured in the ONPG-MUG medium at 36 土1C.The specific enzyme betagalactosidase produced by the total coliform bacteria can decompose the color source substrate of the ONPG-MUG medium, which makes the culture medium yellow; meanwhile, Escherichia coli produces a specific beta-glucuronase to decompose the fluorescent substrate MUG in ONPG-MUG medium and produce characteristic fluorescence. The same principle, the heat tolerance coliform group (fecal coliform group) will decompose the color source substrate ONPG in the ONPG-MUG medium at 44.5 土 0 . 5 °C, making the medium yellow  

Packaging specification

 200 /box
Usage method Qualitative detection Step1: Add reagent in 100ml water sample, dissolve it. and Culture 24h at 36 士 1 ° C step2: Read result Colorless = negative Yellow = total coliform positive Yellow + fluorescence = Escherichia coli positive Note: The heat tolerant coliform group (fecal coliform) needs to be cultured at 44.5 士 0 .5°C 24h, and yellow is positive.     Quantitative detection 1.Add reagent in 100ml water sample, dissolve it.    2.Pour into the 51 or 97 hole detection plate 3.Use LK sealer to seal the detection plate, and cultivate 24h at 36 °C. 4.Results qualitative test was used to check the MPN table count.   Note: The heat tolerance coliform group (fecal coliform group) needs to be cultured at 44.5 士 0.5°C 24h, and the Yellow hole is positive, and the corresponding MPN table count is found.

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